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Lionheart FXTM智能活细胞成像分析系统采用zhuan门为成像分析打造的Gen5TM 3.0ruanjian，keyiwanchengtuxiangdebuhuo、fenxi、zhushijishipinzhizuodenggongneng，jubeilecongjichudetuxiangpaishedaogaoneihanfenxidegexianggongneng，tongshicaozuojianbian，yiyuxuexi，wuxufuzahaoshidepeixun。lionheart fxcaiyongjichenghuasheji，tijixiaoqiao，peiheqiangdadegen5ruanjian，shiqichengweiyikuanzhinenghua、quanxingneng、quanzidongdechengxiangfenxixitong，weinindailaiqiansuoweiyoudehuoxibaojiancetiyan。
1、多种成像模式可选
（1）荧光
yingguangjiancemanzu1.25zhi100beidewujingchengxiangxuqiu，congdibeidezhengtituxiangzhigaobeideyaxibaojiegou，junkeqingxichengxian。yicichengxiangkejinxing4zhongbutongsecaidediejia，bingqieyou17zhongyishangdesecaimokuaikegongxuanze，manzuyonghuduibutongsecaichengxiangdexuqiu。
（2）相差&数码相差
lionheart fxzaimingchangxiajuyouxiangchachengxiangmoshi，keyouxiaotigaochengxiangduibidu，qufenxibaoneijiegouweixiaochayi，baozhengxibaozaiziranzhuangtaixiajinxingpaishe，wuxurenhegudingbiaoji。ershumaxiangchagongnengzekeyizaifeixiangchamoshixiashuzimonixiangchachengxiangxiaoguo，tigaotuxiangduibidu，shiputongmingchangxiadechengxiangxiaoguogengweiqingxi。
（3）明场&彩色明场
lionheart fxketigongmingchangyangpinjiancetongdao，feichangshihezaifeibiaojizhuangtaixiawanchenghuoxibaodefenxishiyan。mingchangtiaojianxiahaikejinxingcaisechengxiang，zhenshizaixianyangpindeyuanyousecai，feichangshiheh&ejimianyizuhuaranseyangpindechengxiang，bingkeyipeihebiotekxianjindetuxiangpinjiejishu，huoquzhengtizuzhiqiepiandetuxiang。
（4）动态成像与视频合成
dangxuyaojinxingzhangshijianhuoxibaodongtaiguanceshi，lionheart fxkeyiweiyonghutigongdongtaipaishe，shoudongmoshixiapaisheshijiankeda7tian，suohuodededuilietupian，keyijinxingshipinhecheng，duiyukuaisudongtaishiyan（rugailiu）yekeyikaiqishishishezhigongneng，huoqulianxushipinwenjian。
2、硬件特点
（1）色彩通道与物镜
caiyongkexuejiledguangyuan/lvguangpiandulimokuaisheji，weimeigesecaitongdaojinxingliangshendazao，guangpuruilijunheng，yingxiangbeijingganjingqingxi，huodedetupianzhilianghengao。6weizidongwujingzhuanpan，kerenyixuanzedibei、gaobei、xiangchawujing，qiehuanqingsongziru，fangdabeishuyou1.25zhi100beiyoujing。
（2）温度、气体与湿度控制
Lionheart FX为活细胞检测所打造，因此优异的活细胞生长环境必不可少，Lionheart FX具有温控系统，并可以控制检测仓内CO2和O2denongdu，zhuanyongdeshidukongzhicangjuyouqitiguanzhugongneng，keyixunsuwanchengqitiguanzhubingjiayibaochi，tongshikeyiwanchengcangneideshidujiankong。
（3）自动加样器与自动操纵杆
dangxuyaoduixibaojinxingjiayanghoushishijianceshi，lionheart fxyouliangzhongzidongjiayangtoukexuan，ceweijiayangtoukeyizaijiayanghouduixibaojinxingchengxiang，shihechangguishengwuxuefenxi；yuanweijiayangtouzekezaijiayangtongshijinxingchengxiang，yongyukuaisushengwuxuefenxi，rulizitongdaohuogailiu。lingwaiyonghuhaikexuanzequanzidongcaozonggan，shiguanceshiyangpindeyidonggengweilinghuoziru。
（4）广泛的耗材兼容性
lionheart fxjuyouguangfandehaocaijianrongxing，cong6-1536kongban、xianweijingbopian、xibaopeiyangmin、xibaopeiyangpingdaoxibaojishubanheqiangshibopian，gaodudiyu1.5yingcundehaocaijunkeshiyong。jidadetuozhanlechanpinzaixibaoshengwuxueyanjiulingyudeyingyongfanwei。
3、强大的软件功能
Gen5TM 3.0 imaging primekeyizidongwanchengtuxiangcaijihechuli，suoyoudetuxianghuoqujunshixianzidonghuacaozuo。zidongdexyzaiwutai、zidongjujiao、zidongpuguangyijizidongdeledqiangdudiaozheng。duiyiqidejingzhunkongzhikeyishixianduijuyouyidinghoududeyangpinjinxingcengqie、diejiahezhengtituxiangpinjie。quanxinshengjidetuxiangfenxigongneng，keyishixiangengweiguangfandetuxiangfenxiyaoqiu，congjiandandexibaojishu、yaqunfenxidaogaoneihanfenxi；conggaozhiliangdetuxiangbuhuo、tuxiangzhushidaoshipinzhizuojunkemanzu。gen5 3.0ruanjiancaozuojiandanyiyushangshou，wuxufuzadepeixunjikeqingsongzhangwo，shiyikuannandedetuxiangbuhuofenxiruanjian。
4、应用领域广泛
lionheart fxzhuyaoyingyongyuhuoxibaochengxiang、xibaopeiyangqc、xibaozengzhiyudiaowang、3dxibaopeiyang、zhongliuqinxi、xinhaozhuandao、ganxibaofenhua、biaoxingfenxi、xibaoqianyiheqinxi、gailiu、zhuanwei、xibaoduxing&xibaohuoxing、sunshangxiufu、xibaotunshi、bopiansaomiao、h&echengxiang、wubiaojihuoxibaofenxideng......
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常规：
孔板类型：6-1536孔微孔板
其他耗材：显微镜玻片，细胞培养瓶（T25、T75），细胞培养皿，细胞计数板，腔室玻片
温度控制：环境温度至40°C，±0.5°C@37°C；并具有抗凝集功能，
气体控制：0-20% CO2控制；1-19% O2控制
环境控制罩：用于顶部避光及环境控制
载物台：高分辨率全自动载物台，分辨率0.1μm，带有接入插口
湿度控制：可选湿度控制仓，带有快速气体灌注功能
软件：Gen5 3.0图像捕获与高级分析软件
尺寸：46.5 cm x 45.5 cm x 35.8 cm（长x宽x高）
重量：26.3Kg
成像：
成像模式：明场，彩色明场，相差及荧光场
成像方式：单色，多色，图像拼接，时间延迟，Z-轴层切叠加，触发模式
光源：高能量、长寿命、科学级LED（可选波长365nm、390nm、465nm、505nm、523nm、590nm、623nm、740nm）
相机：Sony16bit科学级灰阶CCD，125万像素，像素尺寸6.45μm
相机整合：2x2 Binning可选
曝光时间：5ms-4s
色彩通道：DAPI，CFP，GFP，YFP，RFP，Texas Red，CY5，CY7，Acridine Orange，CFP-YFP，FRET，Chlorophyll，Phycoerythrin（PE），Propidium lodide，CY5.5，TagBFP，GFP（Ex）-CY6（Em），RFP（Ex）-CY5（Em），Alexa 568，Ex377/Em647
物镜位：6位全自动物镜转盘
物镜：1.25x，2.5x，4x，10x，20x，40x，60x（空气镜）；60x，100x（油镜）；4x，10x，20x，40x（相差）
图像采集速率：10帧/秒 ，单色，全分辨率；20帧/秒，单色，2x2Binning
图像输出可选：
原始图像：16bit TIFF
保存图像：TIF，JPG，BMP，PNG，EMF，GIF
视频：MP4，WMV
视频制作：实时视频或队列图片合成视频
自动方式：自动聚焦，客户自定义聚焦，自动曝光，自动LED强度
聚焦方式：基于图像的自动聚焦和基于激光的自动聚焦
载物台控制：Gen5软件控制或选配全自动操纵杆控制
加样器：
数目：2个
支持耗材：6-384孔板，细胞培养皿，腔室玻片
分液头选择：
侧位分液头-与成像光路不平行，用于普通细胞生物学分析
原位分液头-与成像光路平行，用于快速动力学分析
分液范围：5-1000μL，1μL步进
分液jing确性：±1μL或2%
分液jing确性：<2% @ 50-200 μL
死体积：<1.65mL
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1、活细胞呼吸与线粒体功能实时监测
呼吸与代谢是细胞正常功能的重要指示剂，可作为研究线粒体功能的参数以及作为触发氧化磷酸化转化为有氧糖酵解的标志因子。LuxcelTMgongsicaiyongteshudeyingguangbiaojitanzhen，keyishishijiancexibaowaihaoyanglv、xibaowaichansuanlvyijixibaoneiyanghanliang，yongyupingguhuoxibaodianzichuandilianhetangjiaojietongliangdehuoxing。
2、高密度细胞排斥法进行细胞迁移分析及成像
shiyancaiyonggaomiduxibaopaichifalaijinxingxibaoqinxidexiangguanyanjiu。oris™ proxibaoqianyifenxifazai96huo384kongbandezhongxinquyufangzhishengwujianrongjiaozhi，peiyangxibaozaijiaozhiwaiweixingchengdancetiebixibao，suizhexibaopeiyangbanzhongxinjiaozhiderongjie，banzhongxinchanshengyigewuxibaofugaideguancequyu。shiyanrenyuankeyicaiyonglionheart fxsuishijiancexibaozaijiarubutongcijijihuoyizhijihoudepaxingqianyiqingkuang，bingduiguancequyuneixibaoshengzhangdeqingkuangjinxingcedinghefenxi。
3、高通量RNAi细胞芯片库分析
caiyongzhuanlidernaichengxiangxinpianban，meizhangxinpianshanghanyou3200gewanquandulidesirnaweidian，meigeweidianshangbaohanlejiedaosirnajinruweidianquyupeiyangxibaodeshiji。suoyouweidianjuzhenpailiebingjuyoubianhao，meigeweidiandaibiaoyigedulidernaishiyan，yincikeyishixianxinpianshigaotongliangrnaichengxiangfenxi。
4、利用3D肿瘤细胞侵袭法进行yao物筛选
quanxinde3dxibaopeiyangjishukeyiyoudaoxibaoanzhaozhengchangdezuzhigoujiahetongxinwangluojinxingshengzhang，daliangyingyongyutiwaideyaowushaixuanshiyan。benwencaiyong3dxibaopeiyangjishu，cushizhongliuxibaoyuqitachengfenxibaobaotuanshengzhangchengweizhongliuyangqiuti，tongguoweikongbandexiangguancaozuo，liyongmingchangjiyingguangchangdefencengchengxiangyudiejiaduizhongliuyizhijidedaixieyucanliujinxingfenxi，bingzai3dxibaotuandejichushangjinxingkangzhongliuqinxiyaowudeshaixuanpinggu。
5、高通量全自动组织芯片扫描成像
zuzhixinpianjishuzaishengwuyixuelingyuyouguangfandeyingyong，baokuoshengwuyangbenku、biaobenhezuzhiyangpincundang、yangpinfenjifenlei、zhiliangkongzhi、fenxishiyanzhongkangtidebiaojiheranseyouhuadeng。lionheart fxsuojuyoudecaisemingchangchengxiang，kemanzuyangpindeziransecaichengxianghejiyuh&eransedeyangpinchengxiang，qiquyusaomiaojipinjiemoshizekemanzuzuzhixinpiandezhengtisaomiaoyuchengxiangxuqiu。qidaozhiyingguangxianweichengxiangmokuaizekeyimanzuyingguangbiaojidezuzhixinpiandetuxiangbuhuo。zhuanyoudebopianshipeiqikeyiwanquanshipeizuzhixinpiandeyangpinbopian，bingkeyihechubanqixianghuzhenghe，jinxinggaotongliangdexinpiansaomiaochengxiangcaozuo。
6、高通量彗星分析
彗星分析是用于评价哺乳动物细胞损伤的一项技术，并可以用于监控多条通路DNA损伤修复的能力。主要通过荧光标记法对电泳细胞片段进行迁移分析。Lionheart FX配合Gen5 3.0分析软件可以高通量完成不同规格玻片及孔板内的彗星电泳图像捕获，并对批量数据进行相关分析。