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1、RTCA DPlus系统
系统组成
xcelligence rtca dplusduogongnengshishiwubiaojixibaofenxiyikeyishixianduigezhongtiebixibaodejiance,zhuyaobaokuoxibaojinrunjixibaoqianyi,xibaozengzhijiance。xibaodeshengzhang,zengzhi,jiance,xibaozhanfujishengzhangjiance,zaishoutijiedaodexinhaotonglujianceguochengzhong,rtca dpxitongdedongtaijiancebaozhenglexibaodeshunshixiangyingjizhangshijianxiaoyingdehuoqu。rtcashishiwubiaojixibaofenxiyidejiancelingminxingheyucexing,juyoushishishujucaijitexing,jiancezhouqideduanqijizhangqijianceyoushi。
技术原理
shishiwubiaojidongtaifenxijishu(rtca,real time cell analysis)shianjielunshengwu(yuanaisenshengwu)dehexinjishu。gaijishucaiyongteshugongyi,jiangweidianjiliezhengzhenghezaixibaopeiyangbandemeigexibaoshengzhangkongdibu,yongyigoujianshishi、dongtai、dinglianggenzongxibaoxingtaihezengzhifenhuagaibiandexibaozukangjiancechuanganxitong。
技术优势
rtcashishiwubiaojidongtaijiancexibaohuoxingheduxing,huoquxibaohuoxingdonglixuetupu,youzhuyuquedingqitajiancefangfa(ruxibaosiwang、xibaodiaowanghuoqitaxibaofanyingguochengzhong)dezuijiajianceshijiandian。rtcajishujiancedeshishixingtigonghuahewuqixiaodejingquexinxi。jiyuzukangdeshishiwubiaojifenxixitong,juyouxialieyoushi:
- CV更低,检测灵敏度更高
- 持续动态监测细胞活性,识别细胞毒性产生的起始时刻,指示zui大效应发生的时间
- 在细胞凋亡及细胞周期等检测中,帮助确定zui佳检测时间
- 在实验初期,既可识别细胞接种数目是否合适,zui大限度地减少数据分析过程中,细胞接种及培养板边缘孔效应的影响
- 一种无需标记、动态监测、整个检测NK细胞介导的、细胞杀伤的方法
2、RTCA DPlus包括两个组成单元
RTCA DPlus信号检测和数据分析单元,以及RTCA DPlus系统操作和数据处理单元。RTCA DPlus信号检测和数据分析单元配套有3个E-Plate 16检测模块,可根据不同使用者的需要进行独立操作。独具匠心的设计,使得RTCA DPlus信号检测和数据分析单元可直接放入CO2fuyuxiangzhong,baozhengleyouhuadexibaopeiyangtiaojian。
检测优势
- 无需标记,对细胞无损伤,在最接近生理状态下进行检测,结果准确度高
- 自动、连续监测,同时检测短期(数分钟)和长期(数周)细胞效应,获取全过程动态信息
- 交叉式电极设计,确保高精确性和高重复性,提供更大的动态检测范围
- 完整细胞效应图谱,提供大量、重要的动态反应信息,具有重要指导意义
- 活细胞质量控制,真正实现自身对照参考
仪器特点
- 持凑型设计,体积小巧,节省空间
- 高灵活性3个独立E-Plate 16模块,可同时满足不同研究者的应用需求
- 读取整个E-Plate 16检测最多只需要4秒,捕获细胞响应信号的准确度高
- 支持从细胞迁移、浸润到细胞毒作用等多种检测应用,功能更灵活
- 最新1.2版本软件,添加网络功能,可对IC50、EC50等数据进行自动计算
- 设备安装简单,即插即用,易维护
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1、RTCA DPlus分析仪
chi cun:24.0cm×26.0cm×22.5cm(w×d×h)(cradlewanquandakai)
zhong liang:4.5kg
ceshixinhao:ceshipinlv10,25,50khz;22mv rms±20%;zhiliupianzhi<5mv
celiangjingdu:±(1.5%ji+1ω)
ceshizhongfuxing:99.2%
dongtaifanwei:10ω-5ω
kaiguandianzu:2ω-5kω
tong xun:usbzhuanrs232(botelv:57600bps)
huanjingwendu:+15℃~+40℃
xiangduiwendu:≤98%,wulengning
2、E-plate 16
chi cun:4.0cm×8.7cm×1.96cm(w×d×h)(hangaizi)
kong jian ju:kongzhongxinjuwei9mm,fuheansi/sbs 4-2004biaozhun
kong rong ji:270μl ± 10μl
kongdizhijing:5.0mm ± 0.075mm
xinhaojiekou:yurtca dp analyzeryazhenjiechu
chuanganqizukang:pbsrongye,10khzceshitiaojianxia17ω ± 5ω
cai liao:shengwuxiangrongbiaomianziwaimiejun
huanjingwendu:+15℃~+40℃
xiangduishidu:≤98%,wulengning
3、CIM-Plate 16(上室)
chi cun:3.99cm×8.69cm×0.97cm(w×d×h)(hanmo)
kong jian ju:kongzhongxinjuwei9mm,fuheansi/sbs 4-2004biaozhun
kong rong ji:180μl ± 5μl
kongdizhijing:5.0mm ± 0.075mm
xinhaojiekou:yurtca dp analyzeryazhenjiechu
jie gou:yucim-plate16xiashizhuangpeishiyong
chuanganqizukang:pbsrongye,10khzceshitiaojianxia17ω ± 5ω
cai liao:shengwuxiangrongbiaomianziwaimiejun
huanjingwendu:+15℃~+40℃
xiangduishidu:≤98%,wulengning
4、CIM-Plate 16(下室)
chi cun:3.99cm×8.69cm×1.94cm(w×d×h)(hanmo)
kong jian ju:kongzhongxinjuwei9mm,fuheansi/sbs 4-2004biaozhun
kong rong ji:162μl ± 3μl
kongdizhijing(oxingquan):6.0mm ± 0.075mm
jie gou:yucim-plate16shangshizhuangpeishiyong
cai liao:shengwuxiangrongbiaomianjiamashexianmiejun
huanjingwendu:+15℃~+40℃
xiangduishidu:≤98%,wulengning
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1、细胞治疗杀伤效果评估
butongdecar-txibaotixianlebutongdeshashangtezheng,qizhongyixiejuyouduanshichengshashangxiaoguo,danbujubeizhangshichengshashangnengli;ertongshijubeiduanshichengjizhangshichengshashangxiaoguodecar-txibaoshiyanjiuxuyaode。
rtcajishushishipingjiabutongbaxiangdecar-tshashangnengli,dedaowanzhengshashangdonglixuequxianjizuijiaxiaobabi,bangzhuyanjiuzhegenggaoxiaolvjinxingcar-tyaowuyanfa。tongshi,rtcajishukeyiyuyingxiangjieguoxianghuzuozheng,fengfuyanjiushouduan。
2、环境毒理评价
gegewumaishoushinongdujuntixianduibeas-2bdexibaoduxing,ic50 0.186mg/ml。
rtcajishushishiwubiaojijiancekongqiwuranwu——wumaikeliduirenyuanfeixibaoduzuoyong,bingjisuanrenyishijiandianic50zhi。
3、材料毒理评价
rtcajiancenamiyanghuaguicailiaoduichoxibaoduzuoyongbingjisuanic50zhi。