zhipuchengxiang（mass spectrometry imaging，msi）jishushiyizhongxinxingdeyuanweibiaomianfenzichengxiangjishu。jiezhuzhipudejiegoufenxihejiandingnengli，nenggouzhunquedejiexiduozhongwuzhizaishengwuzuzhibiaomiandeteyixingkongjianfenbu。

        近年来，MSI技术在医学、药学、生物学等领域获得了极大的关注。然而，现有质谱成像技术仅能对同一平面中的样品进行质谱成像分析，要想获得三维空间信息，则只能依靠图像的三维重建技术和数据归一化处理来实现，极为耗时。
zhendui3d msidejishupingjing，deguojisendaxuedebernhard spenglerjiaoshoutuanduikaifalezuixindezidongjujiaosanweizhipuchengxiangjishu，chengguofabiaozaiguojidingjianzazhinature methods——autofocusing maldi mass spectrometry imaging of tissue sections and 3d chemical topography of nonflat surfaces（nature methods, 2017, 14(12): 1156）。zheishiji“1.4μmchaogaofenbianlvzhipuchengxiangjishu”zhihou，spenglerjiaoshoutuanduideyouyicitupo。gaijishutongguobajiguangsanjiaoceliangxitongzhenghedaoap-smaldi10 msixitongzhong，shixianlexiaoyu10μmdecexiangfenbianlv。ruxiatusuoshi，zidongjujiaomaldizhipuchengxiangxitongnenggouqingxidekeshihuamuxuyepianzhongtangganleihezhileiwuzhidesanweikongjianfenbu（fig.1c），manshilietixichongzhonglinzhileiwuzhikongjianteyixingfenbu（fig.1e, f），yijixiaoshunaobulinzhileiwuzhidezuzhikongjianteyixingfenbu（fig.1g, h）。gaijishudechuxiankezhijieduisanweishengwuyangpinjinxingzhipujiance，qizidongjujiaojishunenggoudadatishengjiancexiaolvhejiancetongliang，bingyouxiaobimianyangpinzhongjiancexinhaodequeshi。

Fig.1 苜蓿叶片、曼氏裂体吸虫和小鼠脑部自动聚焦3D质谱成像。a，苜蓿叶片光学成像图；b，总离子质谱图像；c，3D RGB质谱叠加图，[trifolin + Na]+红色、[MGDG(36:6) + K]+绿色、m/z 594.8937蓝色；d，血吸虫光学成像图；e，3D RGB质谱叠加图，[PC(36:1) + Na]+红色、[PC(34:1) + Na]+绿色、m/z 585.0636 蓝色；f，总离子质谱图像；g，小鼠脑部3D-RGB和2D-RGB质谱叠加图，[SM(d40:2) + K]+红色、[PI-Cer(d38:0) + H]+绿色、[PC(40:7) + K]+蓝色，其中上图为自动聚焦，下图为非自动聚焦。图片来源于Nature Methods，（doi:10.1038/nmeth.4433）