7.29ri，“dishiyijiehuanjinghuaxuedahui”zaihaerbinyuanmanluomu，zhongguogongchengyuanyuanshi、haerbingongyedaxuejiaoshourennanqizaikaimushishangfanfuqiangdiao“chuangxinhuanjingkexue，ditanhuanbaojiankang”zheiyizhuti。cicihuanjinghuaxuedahui，jiyulelingyuneigeweizhuanjiadexueshujiaoliudepingtai，tuijinlehuanjingkexuedefazhan。

       huanjingshiguanxizherenleishengcundeguanjianyinsu。dangjinshehuijingjijigongyedegaosufazhandaozhihuanjingwentiyufayanjun，yinciruhejianshaohuanjingwuran，gaoxiaolvdijiejuexianyouhuanjingwenti，chengweidangxiahuanjingkexuederemenhuati。muqian，zhipuchengxiangjishuyichenggongyingyongyushengwutizhongwuranwujiance，qiekeyiduiwuranwuxiepodeshengwu（liru：banmayu）jinxingzhizhikongjianzuxueyanjiu，yongyuyanjiuwuranwuduishengwushenglizhuangtaideyingxiang。

       jizhifuzhujiguangjiexidianlizhipuchengxiang（maldi msi）zuoweiyizhongxianjindefenzichengxiangjishu，keyikuaisu、jingzhun、zhiguandizhanxianchushengwutineigefenzidekongjianfenbu，huodefenziliang、kongjianweizhijikongjianxiangduilizifengdudengxinxi，yincizaihuanjingwuranwuduishengwutiyingxiangdeyanjiuzhongfahuizheyuelaiyuezhongyaodezuoyong。xiamianfenxiangliangpiantransmit ap-smaldizhipuchengxiangjishuzaihuanjinglingyuzhongdeyingyong。

斑马鱼及大型溞脂质分布变化研究

       jinnianlai，youyushifangdaohuanjingzhongdehuaxuewuzhishulianghezhongleibuduanzengduo，haiyangjidanshuishengtaixitongdewuranchengduyeyuelaiyueyanzhong。qizhong，qinzhixingwuzhishitebiexuyaoguanzhudechengfen，tamenkeyitongguoganraoshuishengshengwudezhizhizuchenglaiyingxiangqishenglishenghuatexing。kaolvdaozhizhiduishengwuzuzhijiegoujigongnengzhiguanzhongyao，neimezhizhimoshidegaibiantongchangyubingliguochengxiangguanyejiubuzuweiqile。yincilejiehuaxuexieporuheyingxiangshuishengshengwuzhongzhizhidezuchenghekongjianfenbujiubiandeyouweizhongyao。deguobailuoyitedaxuedeandreas römppjiaoshoushiyanshiliyongchangyajizhifuzhujiguangjiexidianli（transmit ap-smaldi 5 af）jishu，zhenduibanmayuhedaxingsaozheiliangzhonghuanjingkexueyushengtaidulixuelingyuyingyongzuiweiguangfandeshuishengmoshishengwujianlibingyouhuayangpinzhibeiliucheng，jiangliangzhongshengwudezhizhimoshiyuzuzhihuoxibaodejiepoutezhengxiangguanlian，weijinhouyanjiuwuranwuxiepoxiaqitashengwudezhizhimoshibianhuatigongzhongyaodecankaoyiju。

       图1 斑马鱼神经元和非神经元内脂质分布的MALDI MSI成像。(a，e) 成年斑马鱼矢状位切片经H&E染色显示了鳃、眼(视网膜、晶状体)、脑(端脑、中脑盖、小脑)和肝脏的详细结构。(b) PC(O-32:0)[M+H]+(红色)和PC(30:0)[M+K]+(绿色)的RGB叠加图像。(c) PC(O-32:0)[M+H]+的离子图像显示鳃丝处有高信号强度(白色箭头)。(d) PC(30:0)[M+K]+ 的离子图像显示眼睛晶状体和视网膜处的高信号强度 (白色箭头)。(f) PC(O-32:0)[M+H]+(红色)、PC(O-36:1)[M+K]+(蓝色)和PC(40:6)[M+H]+(绿色)dergbdiejiatuxiang。(g) pc(o-32:0)[m+h]+delizituxiangxianshifeishenjingxingzuzhidegaoqiangduxinhao，lirujirouzuzhi(baisejiantou)。(h) pc(o-36:1)[m+k]+delizituxiang，xianshizhongnaogaihebufenxiaonaodejiegou。(i) pc(40:6)[m+h]+delizituxiangxianshidanaoheyanjingyijiganzang(baisejiantou)xiangduiweizhidefenbu。

 玉米中黄曲霉毒素B1及植物防御代谢物空间分布研究

       weileyingduibulihuahewudecunzai，zhiwukeyishengwuzhuanhuawaiyuanwuzhi、zhuanyimutihuahewujiqidaixiewu，bingzaixibaohuozuzhishuipingshangtongguoqushihuazuoyongduiqijinxinggeli。zheizhongqingkuangtongyangshiyongyuzhenjundusu，jizaizhiwuganranguochengzhongfahuizhongyaozuoyongdezhenjuncishengdaixiechanwu。weilemiaoshuyumiyuhuangqumeidusub1zaizuzhiheqiguanshuipingshangdexianghuzuoyongxiaoying，bernhard spenglerjiaoshoutuanduiliyongap-smaldizhipuchengxiangjishuduiyumizhizhudegen、jing、yejinxingyuanweijiance，bingcongdaixiezuxuedejiaodutantaolehuangqumeidusub1（afb1）yuqiqianzaixiushixingshideshengwuzhuanhua、fenbu、dingweijiqiduijiankangyumizhizhudechuji、cijidaixiesuochanshengdehouxuyingxiang。

       图2 AFB1处理14天后，对照样品和AFB1处理的玉米植株根系切片的AP-SMALDI质谱成像。(a)AFB1 [M+K]+,m/z351.0265，在表皮和皮质细胞中积累；(b)矢车菊素葡萄糖苷[M]+,m/z 449.1076；(c)脱镁叶绿素a [M+H]+,m/z871.5729的空间分布；(d)AFB1处理和对照玉米根系切片的光学图像，主要形态特征标记；(e)RGB叠加图像，AFB1[M+K]+，m/z351.0265（红色），脱镁叶绿素a[M+H]+，m/z871.5729（绿色）和矢车菊素葡萄糖苷[M]+，m/z449.1076（蓝色）；(f)rgbtuxiangyuguangxuetuxiangdiejia。kongjianfenbianlv10μm，295×145 pixels。

cankaowenxian：（1）schirmer e, ritschar s, ochs m, et al. maldi mass spectrometry imaging workflow for the aquatic model organisms danio rerio and daphnia magna[j]. scientific reports, 2022, 12(1): 1-11.

（2）righetti l, bhandari d r, rolli e, et al. unveiling the spatial distribution of aflatoxin b1 and plant defense metabolites in maize using ap‐smaldi mass spectrometry imaging[j]. the plant journal, 2021, 106(1): 185-199.