zhipuchengxiang（mass spectrometry imaging, msi）shiyizhipujishuweijichu，tongguozhipuzhijiesaomiaoshengwuzuzhiyangbenshixianchengxiangdexinxing“fenzixianweijing”。gaijishunenggouzaitongyizhangzuzhiqiepianshangyicifenxishubaizhongfenzidekongjianfenbutezheng。

        maldizhipuchengxiangfenbianlvdetigao（<10μm）yizhishigaijishuyanjiudeyigeredianhepingjing。deguojisendaxuebernhard spenglerjiaoshoutuanduizhiliyudaqiyamaldi（transmit ap-smaldi 10）zhipuchengxiangxitongdeyanzhi，shoucishixianle5μmgaokongjianfenbianlvzhipuchengxiang。qijingmideguangxueshejidadatishenglemsidekongjianfenbianlv，tongshibaozhenglejiancedegaolingmindu。gaixitongyichenggongyingyongyuxiaoshubangguangzuzhizhongzhileifenzi（angew chem int ed, 49(22):3834–3838）、xiaoshunaozuzhizhongzhongliubiaozhiwu（histochem cell biol, (2013) 139:759–783）、xiaoshushenzangzuzhizhongkangzhongliuyaowu（anal bioanal chem, (2011) 401:65–73）、gancaogenjingzhonghuangtongleihezaoganlei（the plant journal, 2014, 80(1):161-171）、sishianwentineizhileiwuzhi（anal. chem. 2015, 87(22), 11309−11316）yijihelaxibaozhongneiyuanxingdaixiewu（anal. chem. 2012, 84, 6293−6297）dekongjianteyixingfenbuyanjiu。

        zuijin，guojidingjianzazhinature methodsbaodaolespenglerjiaoshoutuanduidezuixinyanjiuchengguo—atmospheric pressure maldi mass spectrometry imaging of tissues and cells at 1.4-μm lateral resolution（nature methods, 2017, 14(1): 90-96）。youhuahoudeap-maldizhipuchengxiangxitongzaicijiangkongjianfenbianlvtigaodaole1.4 μm，shixianledanxibaofenzichengxiang。ruxiatusuoshi，chaogaofenbianlvap-maldizhipuchengxiangxitongnenggoufenbiandanxibaoshengwucaolvchongyaxibaozhongzhilei、daixiewuyijitaileiwuzhidekongjiantezhengxingfenbu。gaixiangjishudewenshijiangweikeyangongzuozhedailaiwubiaojijiancedequanxinshijiao，tigonggengweifengfudeyaxibaoshuipingdezuzhikongjianxinxi。

（a）草履虫RGB质谱叠加图像，空间分辨率3µm，[DG(31:0)+NH4]+红色、[PC(34:1)+Na]+ 绿色、[Cer(d35:2)+H]+蓝色；（c）草履虫RGB质谱叠加图像，空间分辨率3µm，[PS(32:1)+H-H2O]+红色、[MG(22:1)+NH4]+绿色、[(Lys3Ala)+H]+蓝色；（e）草履虫RGB质谱叠加图像，空间分辨率2µm，[DG(38:1)+NH4]+红色、[(LysAsnArgLeu/Ile)+H-2H2O]+或[(LysArgValGln)+H-2H2O]+绿色、[PC(P-30:0)+H]+蓝色；（g）轮虫吞噬草履虫的RGB质谱叠加图像，空间分辨率2μm，[PE(35:2)+H]+红色、[PC(34:1)+Na]+绿色；（b, d, f, h）分别为a、c、e、g对应的显微镜光学图像。图片来源于Nature Methods，（doi:10.1038/nmeth.4071）